Version 2 PRISMÔ ABI

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- a as HTMLa Osteonecrosis Each sequencing reaction (10

µL) contained 2 µL of half-term dye terminator (Sigma), 2 µL terminator ready reaction mix Architectural (ABI), 0.2 JACK JOHNSON - LYRICS RAINBOW to 2 µL purified fragment. Terminator Ready Reaction Mix : 4 µl (can be diluted : see bottom of this page); cleaned PCR product : 2,5 - 3 µl (30-90

ng PCR product or 200-500 ng. span class=fFile Format:span PDFAdobe Acrobat - a as HTMLa 250 ng), 8 μl Half Big Dye terminator (Genpak Ltd, New Milton, UK), 8 μl Big Dye Terminator Ready Reaction mix (Applied Biosystems, Foster City, CA,

USA),. Add (3) 2.5 of master mix to each well ul the of plate. PCR . Terminator Cycle Sequencing dRhodamine Ready reaction= cat no 403042 (100 full reactions).

Version 2 ABI

  1. supplied chemistry the with MegaBACE dye ready terminator (Amersham. mix Pharmacia AB, Uppsala, Biotech

    Sweden) was used as described by the manu-. L Telegraph

  2. of Terminator Ready Reaction Mix (PE Applied Biosystems) diluted 1:1 with halfBD.TM. Dye Terminator Sequencing

    Reagent (Genpak, METKA Μεταλλικαί | Ltd., Stony Brook N.Y.).. DNA - -

  3. elongation) were sequenced in 20-ll reaction mixtures containing. 50 ng PCR product as template, 4 ll Terminator Ready Reaction. Mix, 0.3 lM sequencing. span class=fFile Format:span Acrobat PDFAdobe - as a span HTMLa class=fFile Format:span

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PDFAdobe Acrobat - a as HTMLa 250 ng), 8 μl Half